How to Reconstitute Peptides – A Research Guide
How to Reconstitute Peptides – A Research Guide
Disclaimer: This guide is provided for educational and laboratory research purposes only. All NuroLabs Research products are sold strictly for research use only. They are not intended for human or animal consumption.
What Liquid to Use?
Peptides are usually supplied in lyophilized (freeze-dried) powder form and require reconstitution before use in laboratory experiments. The choice of solvent depends on the peptide:
Bacteriostatic Water – The most common option, containing 0.9% benzyl alcohol to inhibit bacterial growth.
Sterile Water for Injection – Sometimes used for shorter-term storage.
Acetic Acid (Diluted) – Required for certain peptides that do not dissolve well in water.
Other Solvents – Some peptides may require specialized solutions depending on their chemical properties.
Always check the stability information for each specific peptide to confirm the correct solvent.
Step-by-Step Guide to Reconstitution
Preparation-
Ensure both the peptide vial and the solvent vial are at room temperature.
Wash your hands and prepare a clean workspace.
Sterilization-
Remove the protective caps from both vials.
Wipe the rubber stoppers of each vial thoroughly with an alcohol swab.
Preparing the Syringe-
Attach a sterile needle to a syringe.
Draw in air equal to the amount of solvent you plan to use (e.g., 1ml of air if reconstituting with 1ml of bacteriostatic water).
Insert the needle into the solvent vial and inject the air. This equalizes pressure and makes it easier to withdraw the liquid.
Withdraw the correct volume of solvent.
Adding Solvent to the Peptide Vial-
Insert the needle into the peptide vial.
Contrary to popular belief, you do not need to inject the solvent slowly against the side of the vial. Injecting too quickly may cause some temporary foaming, but this does not damage the peptide. The foam will settle on its own.
Mixing the Solution-
You do not need to shake the vial. Instead, gently roll or swirl it between your fingers about 10 times until the solution dissolves.
If it hasn’t fully dissolved, let the vial sit for 15–20 minutes. It should finish dissolving on its own.
After it has dissolved, store the vial in a cold environment. Refer to the storage notes for full guidance.
Storage Notes
Unreconstituted (powder) peptides
Store in a refrigerated zone (2–8°C).
For long-term storage beyond 1–2 years, keep in a freezer (–20°C or below).
Only use the freezer option if storing for longer than 1–2 years.
Reconstituted peptides
Store in a refrigerator (2–8°C) unless otherwise specified.
Discard after 28–32 days to maintain stability and integrity.
Note
Always check the stability information for each specific peptide, as some may have different requirements.
Final Thoughts
Correct reconstitution ensures that peptides remain stable and ready for research use. By following careful procedures, using the right solvent, and applying proper sterile technique, you maintain vial purity and uphold the highest research standards.
At NuroLabs Research, we emphasise safety, transparency, and accuracy in providing research grade peptides.
Reminder: All products are intended for laboratory research use only.